The microfluidic library sequencing integrated machine combines electro wetting microfluidics, rapid library construction, and third-generation nanopore sequencing technology to automate the process of sequencing nucleic acid samples into nanopores, achieving "nucleic acid in, results out" and truly achieving "one device, two functions, three-step operation, 4-hour results". The equipment can be used for unknown pathogen detection in emergency situations in fields such as disease control, customs, hospitals, military, agriculture, and the environment. Complete automated library construction within 1 hour at the fastest. The device has a small size, light weight, small space occupation, dual power supply mode, self powered operation time of more than 5 hours, and can be portable or loaded with drones, monitoring vehicles, robots, etc....